Graphene-Based Nanomaterials Modulate Internal Biofilm Interactions and Microbial Diversity

Graphene-based nanomaterials (GBMs), such as graphene oxide (GO) and reduced (rGO), possess unique properties triggering high expectations for the development of new technological applications are forecasted to be produced at industrial-scale. This raises question potential adverse outcomes on living organisms especially toward microorganisms constituting basis trophic chain in ecosystems. However, investigations GBMs toxicity were performed various using single species that helpful determine mechanisms but fail predict consequences observed effects a larger organization scale. Thus, this study focuses ecotoxicological assessment GO rGO biofilm composed diatom Nitzschia palea associated bacterial consortium. After 48 144 h exposure these 0, 0.1, 1, 10 mg.L −1 , their physiology, structure, metabolism communities measured through use flow cytometry, 16S amplicon sequencing, Biolog ecoplates, respectively. The both stimulated growth. Besides, exerted strong growth inhibition from 1 influenced taxonomic composition diatom-associated consortium, increased transiently activity related carbon cycling, with weak diatom. On contrary, was shown exert weaker whereas it more strongly physiology. When compared results literature tests, our suggests diatoms benefited diatom-bacteria interactions able maintain or recover its carbon-related metabolic activities when exposed GBMs.